Authors: Vivian Tong; Stefan Olovsjö, Seco Tools AB, R&D Materials and Technology, 737 82 Fagersta, Sweden. Contact: vivian.tong@extern.tu-freiberg.de
An EBSD map and an SEM image of the same area never quite line up: the beam drifts during the scan, the camera moves between acquisitions, the specimen is tilted. TrueEBSD corrects that, so every pixel of the map and every pixel of the images refer to the same point on the sample.
This is the short version of TrueEBSD on a WC-Co composite — the same five maps and the same four distortions, on a cut-down copy of the dataset, so it finishes in under a minute instead of twenty. Read the full page for what each step means and how to tune it.
This script needs the TrueEBSD toolbox, which is not part of MTEX and is distributed separately under Apache-2.0: https://github.com/vtvivian/mtex-trueebsd
addpath(genpath('<path to mtex-trueEbsd>'))The data
trueEbsdWCCoSmall is the centre half of the full WC-Co field of view, coarsened by four: a 20.4 x 15.3 µm area with WC grains about 12 px across. Small enough to be quick, still large enough for every distortion to be measurable. makeWCCoSmall.m in the TrueEBSD toolbox shows how it was cut, and why simply coarsening the whole field does not work.
As in the full dataset the four SEM images are in ebsd.opt.trueEbsdImgs, and pixSzImg is their pixel size in microns.
TrueEBSD needs to know which way round each picture is stored before it can compare them pixel by pixel. Here it works that out for itself: the map and the images were collected in one session and stored the same way, which is the usual case. The frames page is the case where they were not.
The convention set here only decides which way up the figures come out. It has no effect on the correction.
plottingConvention.default('y↓→x')
mtexdata trueEbsdWCCoSmall
display(ebsd.opt.trueEbsdImgs)ans = plottingConvention (y↓→x)
ebsd = EBSDsquare (y↓→x)
Phase Orientations Mineral Color Symmetry Crystal reference frame
0 89 (0.72%) notIndexed none
1 11180 (91%) W C LightSkyBlue -6m2 X||a*, Y||b, Z||c
2 991 (8.1%) Co-fcc DarkSeaGreen m-3m
3 28 (0.23%) Co-hcp Goldenrod 6/mmm X||a*, Y||b, Z||c
Properties: bc, bs, bands, MAD, quality, oldId
Scan unit : um
X x Y x Z : [10 → 30] x [8 → 23] x [0 → 0]
Normal vector: (0,0,1)
Square grid :96 x 128
source: trueEbsdWCCo, centre half of the field of view, coarsened 4x
madeBy: trueEBSD tools/makeWCCoSmall.m
struct with fields:
fsdB3: [192×256×3 double]
fsdT3: [192×256 double]
fsdT1: [192×256 double]
fsdT10: [192×256 double]
pixSzImg: 0.0795Build the sequence
TrueEBSD does not jump straight from the EBSD map to the reference image. It steps through the images one pair at a time, correcting one kind of distortion at each step — which is why there are four images rather than one. Each pair differs by something simple enough to model, where the map and the final image differ by everything at once.
So the order matters. It runs from the most distorted to the ground truth, and each entry names the distortion between itself and the next one: 'shift-drift' for beam drift plus a rigid offset, 'shift' for the camera moving, 'tilt' for the specimen tilt seen at a different kV, and 'true' where nothing separates the pair because it came from one beam scan. The reference is the last one.
'name' is what each image is called once it is attached to the map at the end, so the result reads as ebsd.fsdT1.
% a light box filter first — cross-correlation dislikes noise
img = ebsd.opt.trueEbsdImgs;
img.fsdB3 = rescale(imboxfilt(img.fsdB3,3));
img.fsdT3 = rescale(imboxfilt(img.fsdT3,3));
img.fsdT1 = rescale(imboxfilt(img.fsdT1,3));
img.fsdT10 = rescale(imboxfilt(img.fsdT10,3));
imgList = createArray(5,1,'distortedImg');
imgList(1) = distortedImg('bc','shift-drift', ebsd, 'name','bcImg', ...
'highContrast',1, 'edgePadWidth',3);
imgList(2) = distortedImg(img.fsdB3, 'true', 'dxy', img.pixSzImg, 'name','fsdB3', 'highContrast',1, 'edgePadWidth',5);
imgList(3) = distortedImg(img.fsdT3, 'shift', 'dxy', img.pixSzImg, 'name','fsdT3', 'highContrast',1, 'edgePadWidth',5);
imgList(4) = distortedImg(img.fsdT1, 'tilt', 'dxy', img.pixSzImg, 'name','fsdT1', 'highContrast',1, 'edgePadWidth',5);
imgList(5) = distortedImg(img.fsdT10,'true', 'dxy', img.pixSzImg, 'name','fsdT10', 'highContrast',1, 'edgePadWidth',3);The job holds the whole workflow, and each step below adds to it. Building it also checks that the sequence really is stored one consistent way round.
job = trueEbsd(imgList)job = trueEbsd (as imported)
image distortion shift residual
1 96 x 128 shift-drift - -
2 192 x 256 x 3 true - -
3 192 x 256 shift - -
4 192 x 256 tilt - -
5 192 x 256 true - -Plot the sequence to check the images really do cover the same area. Note how different the contrasts look — that is why matching is done on edges rather than on grey values.
plot(imgList,'TrueEBSD starting image sequence')
Put everything on one pixel grid
The map is on a 0.159 µm grid and the images on 0.0795 µm. pixelSizeMatch resamples them all onto the finest one, so pixel (i,j) means roughly the same place in each.
Images are interpolated linearly. EBSD data is not: orientations and phase labels have no meaningful average, so the nearest measured point is used.
Nothing has been corrected yet — this is only bookkeeping.
pixSzIn = 0; % target pixel length in microns, or 0 for the smallest present
job.pixelSizeMatch(pixSzIn)using default pixel size of 0.079501 um, minimum from imgList
ans = trueEbsd (pixel size matched)
image distortion shift residual
1 192 x 256 shift-drift - -
2 192 x 256 x 3 true - -
3 192 x 256 shift - -
4 192 x 256 tilt - -
5 192 x 256 true - -
common grid: 192 x 256 at 0.08 µmThe resampled sequence is in job.resizedList.
job.resizedListans = distortedImg (y↓→x)
image pixel frame distortion contrast EBSD
1 192 x 256 0.08 µm y↓→x shift-drift high W C, Co-fcc, Co-hcp
2 192 x 256 x 3 0.08 µm y↓→x true high -
3 192 x 256 0.08 µm y↓→x shift high -
4 192 x 256 0.08 µm y↓→x tilt high -
5 192 x 256 0.08 µm y↓→x true high -Adjust the matching windows
Distortions are measured by cutting both images of a pair into small boxes and cross-correlating each box with its partner. That gives a local shift at each box, and those shifts are fitted to the distortion model.
Two settings control it, both in pixels, so they have to be written after pixelSizeMatch and before calcDistortion:
-
ROISize— the box width, a power of two. Rule of thumb: at least four times the shift you expect. The shifts here are a few pixels, so 64 is comfortable. -
NumROI— how many boxes across and down.
This is also where the page's speed comes from. The matching is the entire runtime, and it costs roughly the number of boxes times the square of the box width, so the defaults — chosen for a full-size map — are finer than this small grid needs.
nRows = size(job.resizedList(1).img,1);
nCols = size(job.resizedList(1).img,2);
for n = 1:numel(job.resizedList)
for m = 1:numel(job.resizedList(n).setXCF)
job.resizedList(n).setXCF(m).ROISize = 64;
job.resizedList(n).setXCF(m).NumROI.x = 16;
job.resizedList(n).setXCF(m).NumROI.y = round(16 * nRows/nCols);
end
end
% the last map is the reference — nothing correlates against it
job.resizedList(5).setXCF(1).ROISize = 0;
% correlate raw values where the images already share contrast, and edge
% transforms where they do not
job.resizedList(3).setXCF(1).xcfImg = 'img';
job.resizedList(4).setXCF(1).xcfImg = 'img';
job.resizedList(5).setXCF(1).xcfImg = 'img';Measure the distortion
These are the pictures that will actually be matched: edge transforms where xcfImg is 'edge', grey values where it is 'img'. A band contrast map and a backscatter image have nothing in common as grey values, but their grain boundaries fall in the same places — which is why edges are the default.
plot(job.resizedList,'TrueEBSD image sequence for cross-correlation','xcf')
'fitErr' re-measures the shifts after each correction and reports what is left over. That residual is how you tell whether it worked: around a pixel or less is good.
If a residual comes out above two pixels, TrueEBSD doubles the box size and tries again, repeating until it comes down or the box outgrows the image. The boxes set above are large enough that this does not happen here — the full WC-Co page undersizes one deliberately to show it.
Steps marked 'true' are skipped: nothing separates that pair, so their shift is taken as zero whatever the residual says.
job.calcDistortion('fitErr')fitting the distortion across 5 maps, 4 hops
hop distortion model ROI shift residual
2→1 shift-drift poly11 64 px 3.73 px
linearinterp 64 px 1.83 px
after fitting 1.61 px
3→2 true not correlated - 0.00 px
not fitted 1.52 px
4→3 shift poly11 64 px 3.18 px
Warning: Iteration limit reached for robust fitting.
after fitting 0.33 px
5→4 tilt projective 64 px 3.63 px
poly11 64 px 0.44 px
poly22 64 px 0.31 px
after fitting 0.31 px
ans = trueEbsd (shifts calculated)
image distortion shift residual
1 192 x 256 shift-drift 1 px 1.6 px
2 192 x 256 x 3 true 0 px 1.5 px
3 192 x 256 shift 3.2 px 0.33 px
4 192 x 256 tilt 0.34 px 0.31 px
5 192 x 256 true - -
common grid: 192 x 256 at 0.08 µmCorrect it
Each map is now moved by the shifts of every step between it and the reference. The reference itself does not move.
Resampling is nearest-neighbour throughout, so no orientation and no phase label is ever invented by averaging two real measurements.
job.undistort
plot(job.undistortedList,'TrueEBSD image sequence after alignment')ans = trueEbsd (undistorted)
image distortion shift residual
1 192 x 256 shift-drift 1 px 1.6 px
2 192 x 256 x 3 true 0 px 1.5 px
3 192 x 256 shift 3.2 px 0.33 px
4 192 x 256 tilt 0.34 px 0.31 px
5 192 x 256 true - -
common grid: 192 x 256 at 0.08 µm
Use the result
Every image is now attached to the EBSD map as a per-pixel property, under the 'name' given earlier. So ebsd.fsdT1 is just another map property, and plot(ebsd,ebsd.fsdT1) works like any other plot — no conversion, and it stays with the map through cropping, gridding and indexing.
Plotting them back onto the map is also the quickest check that nothing came out the wrong way round.
fsdB3 is a colour image and keeps all three channels. Plotting onto a map needs one value per pixel, so it is averaged to grey here.
ebsdOut = job.undistortedList(1).ebsd;
figure
nextAxis
plot(ebsdOut('W C'), ebsdOut('W C').orientations, 'coordinates','on')
title('Undistorted MTEX EBSD map (WC IPF out of screen)','Color','k')
for n = 1:numel(job.undistortedList)
im = ebsdOut.(job.undistortedList(n).name);
if size(im,3) > 1, im = mean(im,3); end
nextAxis
plot(ebsdOut, im, 'coordinates','on')
mtexColorMap gray
title(['Undistorted ' job.undistortedList(n).name],'Color','k')
end
Finish
The map and the images now overlay pixel for pixel, and ebsdOut is an ordinary MTEX EBSD map that happens to carry four SEM images as properties. Anything you would normally do with a map works from here — including turning a thresholded image into a phase, which is what the copper page does with its voids.
Run the full page for the same workflow at full resolution over the whole field of view.